Complementation by Construction

Complementation by Construction

Construction, characterization, and complementation of Rhodospirillum rubrum puf region mutants.

Rhodospirillum rubrum is a facultatively phototrophic bacterium that, under certain growth conditions, forms an intracytoplasmic chromatophore membrane (ICM) housing the photochemical apparatus. The puf operon of R. rubrum encodes protein subunits of the photochemical reaction center and the B880 light-harvesting antenna complex. Mutant strains of R. rubrum were constructed by interposon mutage...

Construction of protein fragment complementation libraries using incremental truncation.

Many proteins can have their peptide backbone cut by proteolytic or genetic means, yet the two fragments can associate to make an active heterodimer. This ‘‘monomer-to-heterodimer conversion’’ is referred to as protein fragment complementation (PFC). Such complementation is the reverse of evolutionary processes in which domains are recruited and fused at the genetic level. Classic examples of p...

Structures Preserved by Schur Complementation

In this paper we investigate some matrix structures on C that have a good behaviour under Schur complementation. The first type of structure is closely related to low displacement rank matrices. Next, we show that for a matrix having a low rank submatrix, also the Schur complement must have a low rank submatrix, which we can explicitly determine. This property holds even if the low rank submatr...

Complementation of gene deletions by cell hybridization.

Overlapping deletions in chromosome 7 of the mouse are responsible for activity deficiencies of various liver-specific enzymes, including tyrosine aminotransferase (TAT). In an effort to elucidate the nature and type of action of the deleted genes, somatic cell hybridization experiments were carried out. Enzyme-deficient liver cells of homozygous mutant mice or normal liver cells of control new...